The authors have declared that no competing interests exist.
Lennert lymphoma (lymphoepitheloid lymphoma) is an extremely rare variant of peripheral T-cell lymphoma, not otherwise specified. Here we report a case of Lennert lymphoma diagnosed in a 57-year-old woman. She had a three-year history of waxing and waning lymphadenopathy with a rapid increase in size in the past four months before presentation. A needle biopsy and a fine needle aspiration were non-diagnostic due to extensive necrosis. The patient underwent a right neck lymph node excisional biopsy which showed the lymph node architecture was effaced by numerous and sometimes confluent clusters of epithelioid histiocytes and infiltration of small lymphocytes. Extensive necrosis was present. Immunohistochemical stains revealed a mixed population of B- and T-cells with the T-cells showing diminished T-cell markers CD3, CD5, and CD7. Flow cytometric analysis detected a small population (7% of total lymphocytes) of CD4-positive T-lymphocytes with loss of CD3, CD5, and CD7 expressions. PCR-based T-cell receptor gene rearrangement studies showed positive results (clonal peaks) in both gamma and beta genes. Stains for microorganisms were negative. The overall findings indicate Lennert lymphoma. To our knowledge, this is the first reported case of Lennert lymphoma with extensive necrosis. The patient is undergoing chemotherapy. The diagnosis of Lennert lymphoma can be challenging, particularly in cases with extensive necrosis. Our case highlights that adequate sampling is important in the investigation of patients with suspected Lennert lymphoma. A careful pathologic examination with ancillary studies including flow cytometry, immunohistochmistry, and cytogenetic and molecular studies leads to the accurate diagnosis.
Lennert lymphoma, also known as lymphoepithelioid lymphoma, is a rare variant of peripheral T-cell lymphoma, not otherwise specified (NOS).
A 57-year-old woman with history of depression presented with diffuse lymphadenopathy of the cervical, axillary, inguinal and left thigh lymph nodes. She first noticed cervical and axillary lymph nodes three years ago that were waxing and waning in course. She decided to seek work-up when her left thigh lymph node began to increase rapidly in size over four months. She denied fevers, chills, or weight loss. Her PET scan showed extensive hypermetabolic lymphadenopathy within the bilateral neck, bilateral axilla, chest, pelvis, left upper thigh, and bilateral inguinal regions; the largest lymph node was located in the left upper thigh, measuring 5.2 cm in greatest dimension, with maximum SUV of 14.4. Her LDH at presentation was 239 U/L (reference range: 98-192 U/L). The left thigh mass underwent 18 gauge core biopsy which showed extensive necrosis surrounded by a rim of lymphoid tissue and was non-diagnostic. A fine needle aspiration of a level V cervical lymph node also yielded non-diagnostic results. Finally, core biopsies from a left groin lymph node using 14 gauge needles and an excisional biopsy of a right neck lymph node were obtained, which provided diagnostic tissues. The two specimens showed similar morphologic and immunohistochemistry features. The H&E stained sections showed the lymph node architecture was effaced by a diffuse lymphohistiocytic infiltrate with extensive geographic necrosis (
The patient was treated with CHOEP chemotherapy (cyclophosphamide, doxorubicin, vincristine, etoposide, prednisone) with marked reduction in the sizes of her lymph nodes on clinical exam. A PET scan was performed after four cycles of chemotherapy and showed marked interval decrease in size and resolution of activity of multi-station adenopathy. After six cycles of chemotherapy, PET scan was repeated and showed a new 6 mm hypermetabolic soft tissue structure in the right masticator space and new hypermetabolic foci of skin thickening in the left axilla and right gluteal region. A skin shave biopsy showed dermal lymphohistiocytic infiltrate consistent with involvement by Lennert lymphoma. The patient is on clinical trial of duvelisib and is undergoing evaluation for autologous stem cell transplant.
Lennert lymphoma was first described by Karl Lennert as a variant of Hodgkin lymphoma.
Peripheral T-cell lymphomas as a whole comprise 6% of all non-Hodgkin lymphomas. They arise primarily in middle-aged adults, more often in male patients.
Lennert lymphoma is characterized by effacement of lymph node architecture with small lymphocytes showing mild cytologic atypia and prominent, confluent clusters of epithelioid histiocytes.
The cell of origin of Lennert lymphoma is still controversial. Some studies showed the proliferating cells were mainly CD4 positive, indicating a helper T-cell origin.8,15 Other studies concluded Lennert lymphomas are often derived from CD8-positive cytotoxic T-cells. It is likely that the neoplastic cells in some Lennert lymphoma cases are of CD4-positive helper T-cell origin while in other cases are derived from CD8-positive cytotoxic T-cells. In our case the lymphoma cells were positive for CD4 by flow cytometry.
It is difficult to make a diagnosis of Lennert lymphoma as the neoplastic lymphocytes are scattered in a background of many benign/reactive small lymphocytes and show minimal cytologic atypia. In addition, histiocytic proliferation is non-specific and can be seen in many reactive and neoplastic conditions. The presence of extensive necrosis in our case made the diagnosis even more challenging because proliferation of histiocytes with extensive necrosis is more commonly seen in infections, inflammations, and other reactive conditions. Clinicians and pathologists should be aware of this entity and the fact that extensive necrosis does not exclude this entity. The key diagnostic clues for this case are the epithelioid histiocyte proliferation, loss of T-cell markers including CD3, CD5, and CD7, and evidence of T-cell clonality by T-cell receptor gene rearrangement studies. The differential diagnoses might include angioimmunoblastic T-cell lymphoma, classic Hodgkin lymphoma, and T-cell/histiocyte-rich large B-cell lymphoma. In this case we did not see the high endothelial venules characteristic of angioimmunoblastic T-cell lymphoma. Although CD30 stained scattered immunoblasts, diagnostic Hodgkin Reed-Sternberg cells were not identified by either morphology or immunohistochemistry. T-cell/histiocyte-rich large B-cell lymphoma was ruled out given lack of CD20-positive large B-cells. Moreover, because of the extensive necrosis, other conditions such as Kikuchi-Fujimoto disease and infections or inflammations should have also been considered as differential diagnoses. In our patient, mycobacterial and fungal cultures were negative, and no microorganisms were detected by Gram, AFB, GMS, and PAS stains.
Very few Lennert lymphoma cases have been studied by cytogenetic analysis. Although aberrations of chromosome No. 3 (trisomy 3, break in q22, or dup q22
In conclusion, as far as we know, this is the first report on Lennert lymphoma with extensive necrosis.
JL and CY identified the case. JL performed the literature search and wrote the first draft of the article. PP wrote the clinical history. JC prepared the figures/figure legends and helped shape the manuscript. CY provided critical feedbacks. XQ supervised this work, performed additional literature search, and created the final version of the article.